Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
The ER/PR PolyDetector DAB HRP Brown Detection System is a non-biotin, 1-step polymeric detection system that allows for the Immunohistochemical detection of Estrogen Receptor and Progesterone Receptor in tissues. The ER/PR PolyDetector DAB HRP Brown Detection System has been developed using a proprietary tandem hyperlabelling technology used to directly label immunoglobulins with enzymes. This ensures consistent and reproducible immunostaining for ER/PR. Included in the kit are Breast Tissue Microarray (TMA) slides of formalin-fixed paraffin-embedded tissues or cells that display 0, 1+, 2+ or 3+ positivity that enable the user to quantify the ER/PR expression in tested samples. The Estrogen and Progesterone Receptor (ER/PR) proteins are nuclear hormone receptors overexpressed in various breast carcinomas. ER and PR strongly stain the nucleus of epithelial cells.
The ER and PR are important regulators of growth and differentiation of the mammary gland. The progesterone receptor (PR) is an estrogen-regulated protein. It has been proposed that expression of PR determination indicates a responsive estrogen receptor (ER) pathway.
Presentation
The ER/PR PolyDetector DAB HRP Brown Detection System contains rabbit ER and PR prediluted antibodies clones RBT11 and RBT22, respectively, prediluted rabbit negative control, a 7-core ER/PR cell line microarray, positive control slides, ImmunoRetriever with Citrate heat epitope retrieval solution, Peroxidase Blocker solution, an Anti-Rabbit Horseradish Peroxidase solution, a DAB Buffer and a DAB Chromogen solution. All the components are buffered and contain proteins, stabilizers and a non-azide anti-microbial.
