Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, Kappa exhibits strong staining of kappa-positive plasma cells andcells that have absorbed exogenous immunoglobulin.
When studying B-cell neoplasms, the determination of light-chain ratios remains the centerpiece. This is sound reasoning because most B-cell Lymphomas express either Kappa or Lambda light chains, where as reactive proliferations display a mixture of Kappa and Lambda-positive cells. If only a single light-chain type is detected, a lympho-proliferative disorder is very likely. Monoclonality is determined by a Kappa-Lambda ratio greater than or equal to 3:1, a Lambda-Kappa ratio greater than or equal to 2:1, or a monoclonal population of 75% or more of the total population. In IgG-dominant immune complex-mediated glomerulonephritis, there are multiple pathological findings that strongly suggest the diagnosis of Lupus Nephritis including immunofluorescence staining for IgG, IgM, IgA, Kappa or Lambda, C3 and C1.
Presentation
The Kappa/FITC is a purified rabbit polyclonal antibody labeled with FITC diluted in a Tris Buffered Saline solution (pH 7.2) containing stabilizing proteins and preserved with sodium azide. It is provided in liquid form.