Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
NeuN (Feminizing Locus on X-3, Fox-3, or Hexaribonucleotide Binding Protein-3) is a neuron-specific protein that is present in most central nervous system (CNS) and peripheral nervous system (PNS) neuronal cell types. NeuN protein distributions are restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brains. However, some neurons fail to be recognized by NeuN at all ages, such as inner nuclear layer retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells.
NeuN antibody is widely used to label neurons since the vast majority of neurons are strongly positive. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of doublecortin, a marker seen in the earliest stages of neuronal development. NeuN was detected in most of the amyloid bodies, is considered a marker of neuronal differentiation in brain tumor and has been found in all major subtypes except pilocytic astrocytoma. NeuN antibody IHC have demonstrated NeuN immunoreactivity in 56% of epithelial neuroendocrine carcinomas (ENEC) (19/34): 4 of 7 (57%) grade 1 ENECs (Carcinoid), 4 of 5 (90%) grade 2 ENECs (atypical carcinoid), and 11 of 22 (50%) grade 3 ENECs (small and large cell neuroendocrine carcinoma).
Presentation
NeuN antibody is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Synonyms: ini-1, anti-ini-1, ini1, anti-ini1,, smarcb1, anti-smarcb1, baf47, anti-baf-47, hsnf5, anti-hsnf5
