Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
The Mouse/Rabbit ImmunoDetector Alkaline Phosphatase/ ALK Scarlet Detection System is a Biotin-Streptavidin-Alkaline Phosphatase Detection System that allows for the
demonstration of antigens in formalin-fixedparaffin-embedded tissue, cryostat sections, cytosmears, and cell preparations. The increased sensitivity of ImmunoDetector AP/ALK
Scarlet Detection System allows for rapid staining procedures without compromises in the quality of stains.
The ImmunoDetector AP Blocker is used to block the endogenous Alkaline Phosphatase enzymes that naturally occur in cells and tissue sections without affecting antigens or
nucleic acids. The Mouse/Rabbit ImmunoDetector Alkaline Phosphatase Detection System is suitable for use with mouse (IgG and IgM) and rabbit primary monoclonal and
polyclonal antibodies. The substrate chromogen is the final step in the detection portion; it enables the antibody-antigen-enzyme complex to be viewed under the light
microscope. This occurs when Alk Scarlet, in the presence of Alkaline Phosphatase, gets deposited at the site of the target antigen, producing a scarlet color that is partially
soluble in organic solvents, and therefore care should be taken when mounting with permanent mounting media (please refer to the recommended permanent mounting
protocol on reverse). The ImmunoDetector AP/ALK Scarlet Detection System kits are universal kits and therefore work equally well with prediluted and concentrated antibodies
from different vendors.
Presentation
The ImmunoDetector Alkaline Phosphatase Detection System contains an Alkaline Phosphatase Blocker solution, a Link of Biotinylated Anti-Mouse and Anti-Rabbit
immunoglobulin solution, a Streptavidin conjugated to Alkaline Phosphatase solution, an ALK Scarletchromogen solution, and an ALK Scarlet Buffer-Substrate solution. All the
components are buffered with stabilizers and a non-azide anti-microbial agent.
