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Improved Signals - Less Hands On Time
Introduction
ZytoDot and ZytoDot 2C products are designed for the detection of aneuploidies, gene amplifications or gene deletions and translocations by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads. The advantages of CISH are:
- Simultaneous observation of tissue morphology and CISH signals.
- Quick and easy interpretation of results comparable to IHC.
- Storage of slides at room temperature - CISH signals are permanent.
- No costly fluorescence microscope needed
Method Description - ZytoDot
Single Color CISH for the Detection of Genomic Alterations
The ZytoDot system uses Digoxigenin-labeled probes which are detected using a Mouse-anti-Digoxigenin antibody. This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody. The enzymatic reaction of DAB leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
Method Description - ZytoDot 2C
2-Color CISH for the Detection of Genomic Alterations
The ZytoDot 2C system uses DIG- and DNP-labeled probe cocktails targeting different genomic sections which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail. These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies. The enzymatic reaction of AP-Red and HRP-Green leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy.
ZytoDot Kits and ZytoDot 2C Kits – Convenient Solutions
- All single color ZytoDot CISH probes can be combined with the ZytoDot CISH Implementation Kit (C-3018-40)
- All dual color ZytoDot 2C CISH probes can be combined with the ZytoDot 2C CISH Implementation Kit (C-3044-10/-40)
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