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Outstandingly Fast and Sensitive CISH
Introduction
The ZytoFast products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The signal intensity of ZytoFast probes is increased even more when using the ZytoFast PLUS Implementation Kits.
Method Description - ZytoFast
The ZytoFast Ig-kappa/Ig-lambda Probe uses oligonucleotide probes tagged with Digoxigenin and Biotin to determine the IGK and IGL clonality at once with a Dual Color CISH-System. Digoxigenin is detected by HRP-conjugated antibodies while Biotin is detected using AP-conjugated streptavidin. The enzymatic reaction of chromogenic substrates, e.g. HRP-Green and Permanent Red, leads to the formation of strong color precipitates that can be visualized by light microscopy.
Method Description - ZytoFast PLUS
The ZytoFast PLUS system uses Digoxigenin-labeled probes which are detected using primary antibodies. These antibodies are detected by polymerized enzyme-conjugated secondary antibodies. The enzymatic reaction of chromogenic substrates, e.g. NBT/BCIP, AEC or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.
ZytoFast - Flexibility that meets your needs
- All Biotin-labeled probes can be combined with a ZytoFast CISH Implementation Kit
- All Digoxigenin-labeled probes can be combined with any of the ZytoFast PLUS CISH Implementation Kits
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