Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
The Mouse/Rabbit PolyDetector Alkaline Phosphatase/ ALK Scarlet System is a one-step polymeric detection system that allows for the demonstration of antigens in paraffin-embedded tissue, cryostat sections, blood smears, cytosmears, and cell preparations. The PolyDetector kits have been developed using an Anti-Mouse and Anti-Rabbit Link for maximum sensitivity. Our proprietary hyperlabeling technology used to directly conjugate immunoglobulins Fab‘ monomeric fragments and AP enzymes to a biopolymer backbone molecule. This ensures excellent cellular penetration which generates consistent and reproducible immunostaining for all types of nuclear, cytoplasmic and membranous antigens, in different types of tissues and cell preparations.
The PolyDetector AP Blocker is used to block the endogenous Alkaline Phosphatase enzymes that naturally occur in cells and tissue sections without affecting antigens or nucleic acids. The increased sensitivity of the Mouse/Rabbit PolyDetector Alkaline Phosphatase/ALK Scarlet Detection System allows for rapid staining procedures without compromising stain quality. The Mouse/Rabbit PolyDetector Alkaline Phosphatase/ALK Scarlet Detection System is suitable for use with mouse (IgG and IgM) and rabbit primary monoclonal and polyclonal antibodies. The Mouse/Rabbit PolyDetector Alkaline Phosphatase/ALK Scarlet Detection System kits are universal kits and therefore work equally well with Tintoprediluted and concentrated antibodies from Bio SB and from different vendors’ primaries, as long as they are optimized.
The PolyDetector Alk Scarlet, in the presence of Alkaline Phosphatase, gets deposited at the site of the target antigen, producing a magenta color that is partially soluble in organic solvents and therefore care should be taken when mounting with permanent mounting media (please refer to the recommended permanent mounting protocol on reverse).
Presentation
The Mouse/Rabbit PolyDetector Alkaline Phosphatase/ALK Scarlet Detection System contains an AP Blocker, Anti-Mouse/Rabbit Immunoglobulin conjugated to Alkaline Phosphatase, an ALK ScarletChromogen and an ALK Scarlet Buffer-Substrate. All the components are buffered with stabilizers and an anti-microbial agent.
