Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
PolyDetector ALK Scarlet Substrate-Chromogen is suitable for use in Immunohistochemical, Immunocytochemical and in situ hybridization procedures using Alkaline Phosphatase Detection Systems. PolyDetector ALK Scarlet Substrate-Chromogen allows for the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations.
The substrate-chromogen is the final step in the detection portion; it enables the antibody-antigen-enzyme (or nucleic acid-probe-enzyme) complex to be viewed under the light microscope. This occurs when ALK Scarlet, in the presence of Alkaline Phosphatase, gets deposited at the site of the target antigen or nucleic acid, producing a red color that is partially soluble in organic solvents and therefore can be mounted with permanent mounting media or using the Bio SB ChromoProtector (BSB 0151 – BSB 0156), Bio SB Fast Chromoprotector (BSB 0327-15 - BSB 0327-1000) or using fast xylene/alcohol dehydration protocol.
Presentation
PolyDetector ALK Scarlet Substrate-Chromogen kit contains an ALK Scarlet chromogen solution, and an ALK Scarlet Buffer-Substrate solution. All the components are buffered contain stabilizers and a non-azide anti-microbial.
