PolyDetector DAB HRP Brown Buffer

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

PolyDetector DAB Brown Substrate-Chromogen is suitable for use in HRP Detection systems and allow for the demonstration of tissue antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. 

The substrate chromogen is the final step in the detection portion; it enables the antibody antigen complex or nucleic acid-probe complex to be viewed under the light microscope. This occurs because DAB acts as an electron donor in the presence of the enzyme horseradish peroxidase; DAB gets oxidized and produces a brown color at the site of the target antigen or nucleic acid.

DAB (3’3’diaminobenzidine) is a chromogen (color forming molecule) that develops into a brown precipitate. DAB forms a permanent record of the results of the stain when coverslipped with an organic based resin, such as Perma Mounter (BSB 0094-BSB 0097) or XyGreen PermaMounter (BSB 0169 – BSB 0174).