PolyDetector HRP Black Kit

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

PolyDetector HRP Black Substrate-Chromogen is suitable for use with peroxidase detection systems and allows for the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody antigen complex to be viewed under the light microscope. This occurs because HRP Black acts as an electron donor in the presence of the enzyme horseradish peroxidase; HRP Black gets oxidized and produces a gray/black color at the site of the target antigen or nucleic acid.

HRP Black is a chromogen (color forming molecule) that develops into a gray/black precipitate. HRP Black forms a permanent record of the results of the stain when coverslipped with an organic based mounting medium, such as Perma Mounter (BSB 0094-BSB 0097) or XyGreen PermaMounter (BSB 0169 – BSB 0174)