PolyDetector HRP Black

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

The PolyDetector HRP Black Substrate-Chromogen is suitable for use with peroxidase detection systems and allows for the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody antigen complex to be viewed under the light microscope. This occurs because HRP Black acts as an electron donor in the presence of the enzyme horseradish peroxidase; HRP Black gets oxidized and produces a gray/black color at the site of the target antigen or nucleic acid. 

HRP Black forms a permanent record of the results of the stain when coverslipped with a ChromoProtector (BSB 0151-0156) and organic based mounting medium, such as PermaMounter (BSB 0094-BSB 0097) or XyGreen PermaMounter (BSB 0169 – BSB 0174). It can also be mounted with media such as AquaMounter (BSB 0090-BSB 0093) but the stain will change to brown within 24 hours. If Fast ChromoProtector (BSB 0327-15 to BSB 0327-1000) used or Fast Dip Xylene and OH rehydration protocol prior Permamounter, the Black Signal will stay for about a 1 month. .

The HRP Black is a chromogen (color forming molecule) that develops into a gray/black precipitate.