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TintoFast Androgen Receptor (BSB-4), MMab

Presentation:

Price: $347.00

SKU:	BSB-3690-3
Antibody type:	Mouse Monoclonal
Clone:	BSB-4
Isotype:	IgG1
Concentrate Dilution:	RTU
Localization:	Nuclear

DescriptionDocumentationAsk a QuestionPrice Details

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA-binding transcription factor which regulates gene expression. However, the androgen receptor has additional functions independent of DNA binding. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and the brain.

TintoFast Androgen Receptor antibody reacts with the androgen receptor and also with the newly-described A form of the receptor. This antibody does not cross-react with estrogen, progesterone or glucocorticoid receptors. Abnormalities in the AR-signaling pathway have been linked to a number of diseases, including Prostate Cancer, Kennedy’s Disease and male infertility.

Presentation

TintoFast Androgen Receptor is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Mohs IHC Procedure

Specimen Preparation of Mohs Frozen Tissues

Embed the specimen in OCT inside a cryostat.
Cut sections at 4-5 µm and mount on a positively charged glass slide such as the Bio SB Hydrophilic Plus Slides (BSB 7028) or in the lower third of the TintoDetector Cap Gap slides (BSB 7006).
Air dry the slide at room temperature for 2 minutes and then incubate the slide at 60 °C for 3 minutes in an incubator or dry bath.
Fix in 100% acetone for 2 minutes at room temperature.

Pretreatment of Mohs Frozen Tissues

Preheat the TintoDetector Incubator to 110°C.
Place TintoDetector Cap Gap slides (BSB 7006) face to face and insert them into the TintoDetector Slide Holder (BSB 7003).
Submerge slides in ImmunoDNA Retriever with EDTA to draw up enough solution by capillary action to cover the tissues.
Heat the slides in a preheated TintoDetector Incubator for 3 minutes.
Transfer slides to room temperature and cool off for 1 min.

Mohs IHC Detection

After HIER, transfer slides to ImmunoDNA washer and let it stand for 1-2 minutes.
For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
Wash slides with ImmunoDNA washer or DI water.
Continue IHC detection protocol. Wash slides between each step with ImmunoDNA washer solution.

Abbreviated Mohs PolyDetector Plus DAB HRP Brown or HRP Green Immunohistochemical Protocol

Incubate with Primary Antibody for 5 min
Wash with Buffer (TBST or PBST)
Incubate with M/R link for 4 min
Wash with Buffer (TBST or PBST)
HRP Label for 4 min.
Wash with Buffer (TBST or PBST)
Prepare
- DAB Brown (1 drop of DAB Chromogen in 1ml of DAB Buffer; mix well)
- or HRP Green (1 drop of HRP Green Chromogen in 1 ml of HRP Green Buffer; mix well)
Incubate with DAB or HRP Green for 1-2 min
Wash with Buffer (TBST or PBST)
Counterstain with Hematoxylin or Nuclear Fast Red for 30 seconds
Wash with Buffer (TBST or PBST)
Mount with AquaMounter or dehydrate the tissue with Fast ChromoProtector then mount with PermaMounter

Step	Mohs PolyDetector Plus HRP Green or DAB 20 Minute Protocol
HIER	3 min.
Primary Antibody	5 min.
1st Step Detection	4 min.
2nd Step Detection	4 min.
Substrate-Chromogen	1-2 min.
Counterstain / Coverslip	Varies

This protocol can also be used with FFPE tissues retrieved with Citrate or EDTA.

Specification Sheets

IVD - CE

Safety Data Sheets

SDS

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Option	SKU	Price
Presentation: 3ml Predilute	BSB-3690-3	$347.00
Presentation: 7 ml Predilute	BSB-3690-7	$635.00
Presentation: 15 ml Predilute	BSB-3690-15	$1,097.00

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TintoFast Androgen Receptor (BSB-4), MMab

Intended Use

Summary and Explanation

Presentation

Mohs IHC Procedure

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