The VisionArray Uracil-DNA Glycosylase is an E. coli derived enzyme intended to be used to prevent carry-over contaminations from previous reactions. This is based on the principle of substituting dUTPs for dTTPs during PCR. Therefore, the PCR amplicon can be "discriminated" from the initial DNA strand. Incubating the PCR mixture with the glycosylase at elevated temperatures under alkaline conditions (during the initial denaturation step) will remove contaminating U-DNA from the sample, thereby, avoiding false positive results.
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