Intended Use
For In Vitro Diagnostic Use
Summary and Explanation
In cutaneous squamous and basal cell carcinoma, Perineural Invasion (PNI) is the infiltration of tumor within the perineural space. PNI is an uncommon manifestation of SCC and BCC but can indicate adverse outcomes including recurrence, metastasis, poor prognosis, and death. During microscopically controlled excision, Immunohistochemistry (IHC) may increase accuracy in assessing the presence of PNI, especially in smaller nerves. IHC may be more accurate than routine sections in diagnosing PNI, with significant implications for patient staging, prognosis, and management. Multiplex IHC may further improve PNI detection bydual staining for cytokeratins (CK AE1/AE3, CK 5/6) and nerve markers (NGFR, SOX-10, NF, etc.).
The PNI Carcinoma MultiDetector HRP/AP Kit is a dual stain that allows for the simultaneous visualization of skin carcinomas and nerve tissue. This kit has been optimized with MultiDetector HRP and AP Labels and contrasting chromogens to clearly differentiate tumor cells expressing High Molecular Weight cytokeratin (CK 5/6 in Scarlet) from nerve cells expressing Nerve Growth Factor Receptor (NGFR in Brown). The kit detects Squamous and Basal Cell Carcinomas and nerve bundles using FFPE tissues or frozen Mohs tissue sections.
Presentation
The PNI Carcinoma MultiDetector HRP/AP Kit contains a mouse monoclonal NGFR and rabbit monoclonal CK 5/6 prediluted antibodycocktail, ImmunoDNA Retriever with Citrate heat epitope retrieval solution, ImmunoDNA Washer solution, Peroxidase Blocker solution, AP Blocker solution, a Mouse MultiDetector HRP and Rabbit MultiDetector APLabel cocktail solution, a DAB Buffer and DAB Chromogen solution, and a ALK Scarlet Buffer and Scarlet Chromogen solution. All the components are buffered and contain proteins, stabilizers, and a non-azide anti-microbial.
| Components |
Catalog No. |
|
BSB-0353-7
7 mL Kit
70 Tests*
|
BSB-0353-15
15 mL Kit
150 Tests*
|
BSB-0353-50
50 mL Kit
500 Tests*
|
| 20X ImmunoRetriever with Citrate |
50 mL |
200 mL |
500 mL |
| MultiDetector Peroxidase Blocker |
15 mL |
2 x 15 mL |
100 mL |
| MultiDetector AP Blocker |
15 mL |
2 x 15 mL |
100 mL |
| NGFR MMab & CK 5/6 RMab Cocktail |
7 mL |
15 mL |
50 mL |
| Mouse MultiDetector HRP & Rabbit MultiDetector AP Label cocktail |
7 mL |
15 mL |
50 mL |
| MultiDetector DAB Buffer-Substrate |
15 mL |
2 x 15 mL |
100 mL |
| MultiDetector DAB Chromogen |
1 mL |
2 x 1 mL |
6 mL |
| MultiDetector ALK Scarlet Buffer-Substrate |
15 mL |
2 x 15 mL |
100 mL |
| MultiDetector Scarlet Chromogen |
1 mL |
2 x 1 mL |
6 mL |
| 20X ImmunoDNA Washer |
200 mL |
2 x 200 mL |
1000 mL |
| Fast ChromoProtector |
15 mL |
50 mL |
200 mL |
| Control Slides |
5 Slides |
5 Slides |
5 Slides |
Abbreviated Immunohistochemical Protocol
