PolyDetector HRP Mulberry

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

The PolyDetector HRP Mulberry Substrate-Chromogen is suitable for use with peroxidase detection systems and allows for the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody antigen complex to be viewed under the light microscope. This occurs because HRP Mulberry acts as an electron donor in the presence of the enzyme horseradish peroxidase; HRP Mulberry gets oxidized and produces a dark purple color tinted with red at the site of the target antigen or nucleic acid. 

HRP Mulberry forms a permanent record of the stain results when coverslipped with a ChromoProtector (BSB 0151-0156) and organic based mounting medium, such as PermaMounter (BSB 0094-BSB 0097) or XyGreen PermaMounter (BSB 0169 – BSB 0174). It can also be mounted with media such as AquaMounter (BSB 0090-BSB 0093) but the stain will fade and crystalize within 24 hours.

The HRP Mulberry is a chromogen (color forming molecule) that develops into a dark purple color tinted with red precipitate.