PolyDetector HRP Yellow

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

PolyDetector HRP Yellow Substrate-Chromogen is suitable for use with peroxidase detection systems and allows for the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues,cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody antigen complex to be viewed under the light microscope. This occurs because HRP Yellow acts as an electron donor in the presence of the enzyme horseradish peroxidase; HRP Yellow gets oxidized and produces a yellow color at the site of the target antigen or nucleic acid. HRP Yellow is particularly useful for dual staining or multiplex IHC or CISH purposes. 

HRP Yellow forms a permanent record of the results of the stain when coverslipped with a ChromoProtector (BSB 0151-0156) and organic based mounting medium, such as PermaMounter (BSB 0094-BSB 0097) or XyGreen PermaMounter (BSB 0169 – BSB 0174). It can also be mounted with media such as AquaMounter (BSB 0090-BSB 0093) but the stain will change to brown within 24 hours.

HRP Yellow is a chromogen (color forming molecule) that develops into a strong yellow color that ranges from bright yellow to gold or brown depending on the concentration of the antigen.