PolyDetector Liquid AEC HRP Ready-To-Use

Intended Use

For In Vitro Diagnostic Use

Summary and Explanation

PolyDetector AEC HRP Red Ready-to-Use Substrate-Chromogen is suitable for use in HRP Detection Systems and allow for the demonstration of tissue antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate chromogen is the final step in the detection portion; it enables the antibody antigen complex to be viewed under the light microscope. This occurs because PolyDetector AEC HRP Red acts as an electron donor in the presence of the enzyme horseradish peroxidase; PolyDetector AEC HRP Red gets oxidized and produces a red color at the site of the target antigen or nucleic acid. 

PolyDetector AEC HRP Red is soluble in organic solvents and therefore should be mounted with water-based mounting media, such as Aqua Mounter (BSB 0090-BSB 0093) or it can be mounted with permanent medium when using the Bio SB ChromoProtector (BSB 0151- BSB 0156).

PolyDetector AEC HRP Red Ready-to-Use Substrate-Chromogen (3-Amino-9-ethylcarbazole) is a chromogen (color forming molecule) that develops into a red precipitate.