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Navigate:  StoreFront / Immunofluorescence / Direct Immunofluorescence Antibodies
Above:  IF of IGA on a Frozen Kidney Tissue


Overview & Features

Direct immunofluorescence uses a single, primary antibody, chemically linked to a fluorophore or fluorochrome. The primary antibody recognizes the target molecule (antigen) and binds to a specific region called the epitope. The attached fluorophore can be detected via fluorescent microscopy, which, depending on the fluorophore used, will emit a specific wavelength of light once excited. Direct immunofluorescence, although somewhat less common, has notable advantages over the indirect procedure. The direct detection of the antigen by a primary antibody directly labeled with a fluorochrome reduces the number of steps in the procedure, saving time and reducing non-specific background signal.

  • High Affinity Purified Antibodies Conjugated to FITC.
  • Highly Sensitive and Specific.
  • Low Background.
  • Reduction in time compared to InDirect labeling.
  • Validated for Transplantation and Autoimmunity Studies.

 

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Direct Immunofluorescence Antibodies

 
 
 
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